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. 2018 Nov 10;13:9. doi: 10.1186/s13008-018-0042-4

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Kif18 localization after taxol or nocodazole treatment. a Double staining of MI oocytes with an anti-Kif18a antibody (red) and an anti-α-tubulin antibody (green). Oocytes were counterstained with DAPI to visualize DNA (blue). Kif18a mainly localized on the meiotic spindle. b Subcellular localization of Kif18a after taxol treatment during mouse oocyte meiotic maturation. Arrows indicated asters. Green, α-tubulin; red, Kif18a. c Subcellular localization of Kif18a after nocodazole treatment during mouse oocyte meiotic maturation. Green, α-tubulin; red, Kif18a. Scale bar: 20 μm