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. 2018 Nov 10;13:9. doi: 10.1186/s13008-018-0042-4

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Spindle assembly and chromosome alignment demand hypoacetylation of tubulin K40 during meiosis. a Typical images of MI control oocytes, Kif18a siRNA oocytes and Kif18a siRNA + tubulin K40R mRNA-injected oocytes. Green, α-tubulin; red, DNA. b The incidence of abnormal spindles and misaligned chromosomes in control oocytes, Kif18a siRNA oocytes and Kif18a siRNA + tubulin K40R mRNA-injected oocytes. Data are presented as mean percentage (± SEM) of at least three independent experiments. *p < 0.05; scale bars: 20 μm