Fig. 4.

Knockdown of EGFR suppresses recovery from cisplatin treatment. a EGFR silenced TCCSUP cells (siEFGR) or control TCCSUP cells (siCtrl) were challenged with cisplatin treatment. TCCSUP cells were incubated with cisplatin (10 µM) and siRNAs for 48 h and then re-treated with cisplatin alone for an additional 6 h. After, the cisplatin was removed from the culture media and cells were incubated in normal growth medium for 24 h. b Cell viability was measured using MTT assay. Cell viability levels of three wells of transfected cells were determined. The graph was plotted as %, compared to control, no cisplatin treatment in siCtrl group (± SD). *P < 0.05 (Student’s t-test). All experiments were done in at least triplicates