Table 1.

Cellular Trafficking of WT Rho and Rho Mutants

	with vehicle			with 5 μM 9-cis-retinal
	relative surface immunostaininga	relative intracellular immunostaininga	relative total immunostaininga	relative surface immunostainingb	relative intracellular immunostainingb	relative total immunostainingb
WT	−	−	−	1.27 ± 0.02	1.38 ± 0.01	1.33 ± 0.01
V209M	0.45 ± 0.01	0.75 ± 0.03	0.61 ± 0.02	2.7 ± 0.1	1.60 ± 0.06	1.95 ± 0.03
P215L	0.02 ± 0.01	0.68 ± 0.01	0.38 ± 0.02	−c	1.2 ± 0.2	1.2 ± 0.2
F220C	0.86 ± 0.09	1.01 ± 0.04	0.95 ± 0.05	1.6 ± 0.1	1.4 ± 0.1	1.5 ± 0.1
C222R	0.01 ± 0.01	0.72 ± 0.05	0.40 ± 0.02	−c	1.09 ± 0.09	1.11 ± 0.09

^aIntensity measurements were normalized relative to the corresponding value of WT. Numbers reflect the average value from three biological replicates, and error values reflect the standard deviation.

^bIntensity measurements in the presence of 9-cis-retinal were normalized relative to the corresponding value for each mutant in the absence of 9-cis-retinal. Numbers reflect the average value from three biological replicates, and error values reflect the standard deviation.

^cReliable estimates of these ratios could not be determined because of the low intensity values associated with these measurements.