Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 8;39(43):3879–3892. doi: 10.1093/eurheartj/ehy249

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018. Published by Oxford University Press on behalf of the European Society of Cardiology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Calcium handling in hypertrophic cardiomyopathy lines by optical mapping. (A) A red-genetically encoded calcium indicator expression cassette was engineered into AAVS1 of MYH7-mutant human pluripotent stem cell via nickase CRISPR/Cas9 editing. (B) α-Actinin/red-genetically encoded calcium indicator/DAPI-immunostained human pluripotent stem cell-cardiomyocytes post-gene editing demonstrates expression of the red-genetically encoded calcium indicator calcium sensor (Bar = 50 μm). (C) Optical mapping of R453C-β-myosin heavy chain REBL-PAT cardiomyocytes (n = 10) enabled quantification of (D) beat rate, (E) DAD-like abnormal events, and (F) signal amplitude (correlated with increased systolic calcium peak). Treatment of gene-edited lines with 1 μM nifedipine (n = 5) is in (G, H). Impact of 1 μM ranolazine (n = 6) on red-genetically encoded calcium indicator signal amplitude in (I) and frequency of DAD-like events in (J). Data, mean ± SD. P-values, one-way ANOVA test + Dunnett’s correction.