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. 2018 Oct 11;11(5):1156–1170. doi: 10.1016/j.stemcr.2018.09.004

Figure 1.

Figure 1

A Combination of Factors Induces iDALs in the Adult Mouse Striatum

(A) In vivo reprogramming by SOX2 and VPA is not sufficient for iDALs. TH+ somas were not detected in striatal regions injected with SOX2 virus and treated with VPA when analyzed at 8 wpi (weeks post injection of virus; n > 3 mice). Induced DCX+ neurons were used as controls for SOX2-dependent in vivo reprogramming activity. Cell nuclei were counterstained with Hoechst 33342 (Hst). Scale bar, 20 μm.

(B) Confocal images of iDALs by the indicated factors at 8 wpi. Enlarged views of the boxed regions are also shown. Nuclei were counterstained with Hst. Scale bars, 20 μm.

(C) Quantification of iDALs at 8 wpi (mean ± SD; n = 3 mice per group; group effect by one-way ANOVA: F(10,22) = 7.345 and p < 0.0001). N, NURR1; F, FOXA2; L, LMX1A; DA3, a combination of NURR1, FOXA2, and LMX1A.

(D) A time course analysis of iDALs induced by SOX2+DA3+VPA (mean ± SD; n = 3–4 mice per group; time effect by one-way ANOVA: F(5,14) = 101.7 and p < 0.0001). wpi, weeks post injection of virus.

See also Figures S1 and S2.