Figure 2.

iDALs Do Not Pass through a Proliferative Progenitor Stage

(A and B) iDALs are not traced by BrdU incorporation. Mice were treated with BrdU in drinking water (A) or through twice daily intraperitoneal injections (B) for the indicated durations. Representative confocal images are shown (n = 0 BrdU⁺TH⁺ cells from three mice at each condition). dpi, days post injection of virus; wpi, weeks post injection of virus; IHC, immunohistochemistry. Scale bars, 20 μm.

(C) iDALs do not pass through a progenitor state. Induced neural progenitors were traced in Ascl1-CerER^T2;Rosa-tdT mice after tamoxifen (Tam) injections (n = 0 tdT⁺TH⁺ cells from a total of four mice). Scale bars, 50 μm (in lower magnification view) and 10 μm (in higher magnification view).