FIG 2.

Temperature does not affect the relative copy number of pHERD30T in PA14. PA14 harboring pHERD30T, the empty vector backbone for the pCR2SP1 seed plasmid, was grown at 37, 30, 23, and 15°C on LB agar supplemented with gentamicin (50 μg/ml). The copy number of plasmid DNA relative to chromosomal DNA was measured by qPCR of total DNA using primers for pHERD30T and rpoB. Error bars represent standard deviation (SD) from n = 3 replicates (P = 0.1752, one-way analysis of variance [ANOVA]). ns, not significant.