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. 2018 Nov 13;9(6):e02135-18. doi: 10.1128/mBio.02135-18

FIG 2.

FIG 2

Pas3 regulates cryptococcal cell fusion and filamentation. (A) Colony morphology of the cross between α isolates (WT H99 and PAS mutants) and WT KN99a strain on V8 medium at 22°C in dark for 14 days. (B) Cell fusion products of the indicated crosses at 48 h postcoculturing on V8. (C) WT XL280 and the pas3Δ mutant (OD600 = 3) were spotted on V8 medium to test self-filamentation during unisexual development. (D) The bilateral mating of the wild-type partners (XL280α × XL280a) and the pas3Δ mutants (pas3Δα × pas3Δa) on V8 medium at 22°C in dark for 10 days.