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Thermal shift assays of P. putida KT2440 McpU-LBD against a library of ligands. Shown are the individual Tm changes caused by 95 compounds (Biolog array PM3B) that can serve as nitrogen sources. The inset shows the unfolding curves of McpU-LBD when free from ligand (continuous line) and in the presence of agmatine (dotted line) and histamine (dashed line).
