FIG 5.

Nucleotide exchanges in the loop affect target regulation. (A) Schematic drawing of the mutated PmaR variant with four exchanged nucleotides (marked in blue). (B) Northern blot analysis of PmaR from stationary phase. Transcript levels were determined in the wild type and ΔPmaR mutant supplemented with an empty vector as well as in ΔPmaR mutant complemented with plasmid-derived wild-type PmaR (Comp) and the mutated variant (Mut). (C and D) Northern blot analysis of targets from exponential (C) and early stationary (D) phase. Transcript levels were determined in the wild type and different ΔPmaR mutant strains as described above. Ethidium bromide-stained 16S rRNA served as a loading control.