FIG 8.

PmaR influences transcript stability of target mRNAs. Northern blot analysis of murB (A) and atu3504 (C) in wild type and the ΔPmaR mutant after the addition of rifampin. Ethidium bromide-stained 16S rRNA served as loading control. Transcript half-lives (t_1/2) of murB (B) and atu3504 (D) were calculated by pixel counting from three biological replicates. (E) Transcript stability of different target mRNAs in wild type and ΔPmaR mutant. Half-lives were calculated from at least two biological replicates.