Figure 1.

Hematopoiesis in hiPSC-Derived Teratomas Is Improved by Steady Cytokine Supply in NSGS Mice and by Co-injection of HUVECs

(A) Exemplary flow cytometry (FC) detecting hematopoietic marker CD45 and progenitor marker CD34 in a teratoma generated in NSG mice.

(B) Immunohistochemistry on teratomas generated in NSG mice.

(C) FC summary of hematopoietic populations in teratomas generated with or without hematopoietic supporter cells. Median, quartiles, and outer values are depicted.

(D) Summary of FC analyses of teratoma hematopoietic populations generated in NSGS mice that express IL-3, GM-CSF, and SCF (median, quartiles, and outer values).

(E) Summary of FC results of teratoma samples generated with co-injection of OP9 or HUVECs expressing DLL1, DLL4, or WNT3A (mean and SD).

(F) Fold change of all CD45⁺ cells in teratomas generated with hiPSC and different supporter cell types in NSG or NSGS mice. The median CD45⁺ population of teratomas generated in NSG with hiPSC + OP9 was set to a value of 1. Graph depicts median and range.

Statistics of (C), (D), and (F) were calculated by Kruskal-Wallis and Dunn's multiple comparisons tests (^∗p < 0.05).