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Characterization of iPSCs
(A) ICC showing positivity of iPSC lines for the stem cell markers SSEA4 and OCT4. Scale bar, 75 μm.
(B) Karyotype analysis of iPSC lines aimed to exclude major genetic rearrangements due to the reprogramming process.
(C) qPCR demonstrating high expression levels of stem cell-related genes OCT4 and NANOG in iPSCs compared with fibroblasts.
Data are expressed as means ± SEM.
