Figure 2.

Characterization of iPSC-Derived Dopaminergic Neurons: First Part

(A) ICC showing positivity for the neuronal marker TUJ1 and the catecholaminergic marker TH in the majority of iPSC-derived neurons at 35 DIV. Scale bar, 50 μm.

(B) ICC showing positivity for the neuronal marker MAP2 in the majority of iPSC-derived neurons at 50 DIV. Scale bar, 75 μm.

(C) qPCR demonstrating high expression levels of the neuronal markers TUBB3 and MAP2 and of the catecholaminergic marker TH in iPSC-derived neurons at 35 DIV compared with fibroblasts and iPSCs.

(D) Western blot showing high protein amount of the neuronal marker TUJ1 in iPSC-derived dopaminergic neurons at 35 DIV compared with fibroblasts. AT, affected twin; UT, unaffected twin.

Data are expressed as means ± SEM. See also Figure S1 and Table S1.