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. 2018 Oct 16;9:1519. doi: 10.3389/fpls.2018.01519

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Copyright © 2018 Henke, Junker, Neumann, Altmann and Gladilin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Dependency of the PC registration on plant growth. (A) Median success rates of single-step PC registration of FLU/VIS images for three plant age/growth phases of Arabidopsis (top-view), wheat and maize (side-view) phenotyping experiments including young (1. phase), intermediate stage (2. phase) and adult shoots (3. phase). Reduced success rate of PC registration is caused by a more frequent occurrence of non-specific shape of young wheat and maize shoots in side-view from certain rotation angles. (B) Examples of FLU/VIS images of young and adult maize shoots taken from different rotation angles. The redundant shape (“thin vertical line”) of the young maize shoot in the FLU/VIS image pair taken from the rotation degree 45° does not exhibit unique spectral characteristics causing a failure of the PC registration.