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. 2018 Oct 25;11(5):1257–1271. doi: 10.1016/j.stemcr.2018.09.014

Figure 7.

Figure 7

Temporal Tracing of PAX6 Expression Cells Identifies their Sequential NE and Dorsal NP Identities

(A) Schematic diagram for generating PAX6-CreERT2 lines. Primer sets for genomic DNA PCR are labeled with arrows.

(B and C) Genomic DNA PCR results showing recombination of CreERT2 at the ATG region of PAX6 (B) and CAG-LSL-GFP (C) cassettes within the AAVS1 intron.

(D) Immunolabeling of GFP, PAX6, Cre, and NKX2.1 in dorsal and ventral NPs at day 17 in PAX6-CreERT2/AAVS1-LSL-GFP H9 hESC line treated with 4-OHT through days 8–10. Scale bar, 100 μm.

(E) Immunolabeling of GFP, PAX6, Cre, and NKX2.1 in dorsal and ventral NPs at day 17 in PAX6-CreERT2/AAVS1-LSL-GFP H9 hESC line treated with or without 4-OHT through days 15–17. Scale bar, 100 μm.

See also Figure S6.