Figure 5.

Distinct Cytokine Stimulation of hiPSC-Derived Astrocytes Triggers Specific Secretion Profiles

Cytokine secretion was measured in the supernatant of hiPSC-derived astrocyte cultures following stimulation with MS-associated cytokines for 5 days and compared with untreated cells (Untreated). Concentrations were measured by multiplexed bead-based immunoassay (Luminex). Data are presented as a heatmap of concentrations (pg/mL) going from 0 to the highest value measured for each cytokine after outlier exclusion (calculated with the ROUT method on GraphPad Prism 7, in dark red on the heatmaps). Conditions of stimulation are stacked in columns and cytokines analyzed in lines. For each cytokine analyzed, each of the six horizontal lines represents one study subject, starting with the three HCs (light green) then three MS patients (dark green). Each result represents the mean of two replicates of one experiment performed on one hiPSC line. Data were analyzed with a paired Friedman test (ANOVA) followed by a Dunn's multiple comparison test to assess statistical significance compared with the untreated condition (^∗p < 0.05; ^∗∗p < 0.01; ^∗∗∗p < 0.001; ^∗∗∗∗p < 0.0001).