Figure 6.

Characterization of Definitive Hematopoietic Potential of the CD31/CD235a Populations

(A) T lymphoid potential displayed by the indicated populations after culture on OP9-DLL1. After 1 month, cells were harvested and stained for CD45, CD4, and CD8 expression. Flow cytometric analyses are representative of three independent experiments. Stroma cells were excluded based on their high GFP expression as seen on the control OP9-only culture dot plot on the right.

(B) Quantification and statistical analysis of the total number of lymphoid cells obtained in each experiment. Differences between each population were analyzed by two-way ANOVA. Error bars indicate the SEM from three independent experiments. Adjusted p value using Turkey multiple comparison test: ^∗∗∗p ≤ 0.0006.

(C) Globin expression in cells of hematopoietic colonies obtained from the indicated populations after 2 weeks in methylcellulose culture. Quantification and statistical analysis of the ratio between gamma γ1/2 and epsilon ɛ globin expression analyzed by RT-PCR. Error bars indicate the SEM from three independent experiments. Adjusted p value using SIDAK's multiple comparison test: ^∗p = 0.0326, ^∗∗p = 0.0028, ^∗∗∗p = 0.0002.