Figure 4.

Induced DNA Damage in E12.5 and E14.5 Fancg^−/− HCs and HSCs without Apoptosis or Cell-Cycle Changes

(A) MFI of γH2AX^pos cells in total, HC (CD45), and HSC (34K or SA) in E12.5 PL (n = 3), FL (n = 4), and E14.5 FL (n = 3, and each dot represents an individual mouse), measured by flow cytometry.

(B) Histograms of G₀, G₁, S, and G₂/M phases of the cell cycle determined after Hoechst staining, for Fancg^−/− and WT HC, and HSCs of E12.5 FL and Pl and E14.5 FL. Dots of same colors are related to the same experiment.

Error bars correspond to standard deviation. Gray bars, WT; white bars, Fancg^−/−. ^∗p < 0.05; NS, not significant.