Figure 2.

Expanded Hematopoietic Progenitor Cell Compartment in Prtn3^−/− Bone Marrow Is Functionally Active.

(A) Quantification of in vitro progenitor cell activity as demonstrated by colony-forming cell assays using BM cells (n = 9 per group).

(B) Quantification of in vitro progenitor cell activity as demonstrated by colony-forming cell assays using splenocytes (n = 3 per group).

(C) Representative images of WT and Prtn3^−/− colonies from colony-forming cell assays. Scale bar, 100 μm.

(D) Quantitative analysis of colony sizes from WT and Prtn3^−/− colonies. The sizes of at least ten colonies were measured per sample (n = 6 per group).

(E) Scheme of the experimental setup for the competitive bone marrow transplantation.

(F) Representative FACS dot plots for myeloid cells, B cells, and T cells showing the reconstitution capacity of different donors in the recipient mice.

(G) Quantitative analysis of the distribution of cells in the recipient mice at 8 weeks post transplantation (n = 4 per group). Results are representative of two independent experiments.

All values shown are means ± SEM. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, by unpaired, 2-tailed Student's t test.