Figure 3.

The Expanded Hematopoietic Progenitor Cell Population in Prtn3^−/− Bone Marrow Provides Faster Recovery and Increased Survival after Hematopoietic Injury

(A) The experimental setup used to analyze hematopoietic recovery after challenge with sublethal irradiation (4 Gy).

(B) Total white blood cell counts in WT and Prtn3^−/− mice before and after sublethal irradiation (n = 5–9 per group).

(C) Neutrophil, lymphocyte, monocyte, eosinophil, and basophil cell counts in WT and Prtn3^−/− mice before and after sublethal irradiation (n = 5–9 per group).

(D) The number of BM mononuclear cells per one femur in WT and Prtn3^−/− mice before and after sublethal irradiation (n = 5–9 per group).

(E) Representative FACS dot plots for identifying the frequencies of neutrophils, T cells, and B cells in the BM.

(F) Quantitative analysis of neutrophils, T cells, and B cells per one femur in WT and Prtn3^−/− mice before and after sublethal irradiation (n = 5–9 per group).

(G) Quantitative analysis of LK cells per one femur in WT and Prtn3^−/− mice before and after sublethal irradiation (n = 5–9 per group).

(H) Scheme of the experimental setup for survival analysis after challenge with 7.8 Gy irradiation and the survival curves of WT and Prtn3^−/− mice receiving 7.8 Gy irradiation (n = 23 for WT and n = 36 for Prtn3^−/−).

All values shown are means ± SEM. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, by unpaired, 2-tailed Student's t test (A–G) and log rank (Mantel-Cox) test for survival curve (H). See also Figure S2.