Figure 4.

Elevated Hematopoiesis Caused by PR3 Deficiency Is an Intrinsic Feature of LSK Cells

(A) Scheme of the experimental setup used to compare WT and Prtn3^−/− LSK cells by competitive transplantation.

(B) Peripheral blood cell counts (n = 4 per group).

(C) Representative FACS plots showing WT and Prtn3^−/− donor-derived LSK and LK cells in recipient mice are shown in the top panels. Numbers denote the frequency of specific cell populations among the parent gate. The frequency of WT and Prtn3^−/− donor-derived LSK and LK cells among total BM cells is shown in the middle panels. The percentage of LSK and LK cells from individual donors is shown in the bottom panels (n = 4 per group).

(D and E) Quantitative analysis of the origin of cells in recipient mice at 16 weeks post transplantation in (D) the BM and (E) peripheral blood (n = 4–7 per group).

All values shown are means ± SEM. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, by unpaired, 2-tailed Student's t test. See also Figure S3.