. 2018 Oct 31;285(1890):20182157. doi: 10.1098/rspb.2018.2157

Table 1.

New marker genes, primers and probes for the S. velum symbiont used in the PCR, qPCR and ISH experiments.

	target	gene	name		sequence (5'–3′)	references
PCR primers	S. velum symbionts	rhlE (JV46_09660)^a	SvrhlE	112F	GGACGGGATATCATGGGTGG	this study
	S. velum symbionts	rhlE (JV46_09660)^a	SvrhlE	717R	CAGCCAGGAGAGGAGTTCAC	this study
	S. velum symbionts	gyrB (JV46_17100)	SvgyrB	856F	GCCCTGACAAGGACGCTCAACC	this study
	S. velum symbionts	gyrB (JV46_17100)	SvgyrB	2312R	GCATCAGCAGCAACAGCATCTTCG	this study
	S. velum mitochondria	atp6	Svatp6	70F	TGAGCAGGAACTTTATGGGTT	this study
	S. velum mitochondria	atp6	Svatp6	522R	AAGAACCACATGCCCAGCTC	this study
	bacteria (universal)	16S rRNA V4 region	EUB	16SV4F	GTGCCAGCMGCCGCGGTAA	Caporaso et al. [30]
	bacteria (universal)	16S rRNA V4 region	EUB	16SV4R	GGACTACHVGGGTWTCTAAT	Caporaso et al. [30]
ISH probes	S. velum symbionts	16S rRNA (47–76 bp)	Svsym47		CCCGAAGGTATTTTCGCGTTCGACTTGCAT	this study
	S. velum symbionts	none; reverse complement of Svsym47	NONSvsym47		ATGCAAGTCGAACGCGAAAATACCTTCGGG	this study
	eubacteria (most)	16S rRNA (338–355 bp)	EUB338		GCTGCCTCCCGTAGGAGT	Amann et al. [29]
	eubacteria (most)	none; reverse complement of EUB338	NON338		ACTCCTACGGGAGGCAGC	Amann et al. [29]
	eukaryotes (most)	18S rRNA gene (516–531 bp)	EUK516		ACCAGACTTGCCCTCC	Amann et al. [29]

^aMarker gene identified with SMD.