| Step | Temperature | Time | Notes |
| 1. | 94 °C | 3 min | |
| 2. | 94 °C | 30 s | |
| 3. | 50 °C | 30 s | |
| 4. | 70 °C | 2 min | 1 min/kb to be amplified, round up |
| Return to step 2 24 times | |||
| 5. | 70 °C | 5 min | |
| 6. | 12 °C | for ever | final hold |
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