Fig. 2.

Effects of TRRAP knockdown on cell cycle and cell death in A2780-SP cells. (A) Flow cytometry was used for cell cycle analysis (right) after infecting A2780-SP cells with sh-control or sh-TRRAP lentivirus. The percentages of G01/G1, S, and G2/M Phases were counted. Representative data from four independent experiments are shown. (B) Percentages of apoptotic (sub-G1 phase) A2780-SP cells. Results are presented as mean ± SD. *P < 0.05 (n = 4). (C) The effects of TRRAP knockdown on the levels of cell cycle- and apoptosis-associated genes in A2780-SP cells. The protein levels of p21, CDK4, cyclin D3, PARP-1, and GAPDH were probed through Western blot analysis. Representative data from three independent experiments are shown.