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. 2018 Oct 31;51(10):514–519. doi: 10.5483/BMBRep.2018.51.10.042

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Copyright © 2018 by the The Korean Society for Biochemistry and Molecular Biology

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3 — Role of TRRAP in the expression of NANOG in CSCs. (A) Effects of TRRAP knockdown on the expressions of stemness factors in A2780-SP cells. The mRNA levels of OCT4, SOX2, and NANOG were assessed after silencing TRRAP in A2780-SP cells. (B) RT-PCR analysis results for OCT4, SOX2, and NANOG mRNA levels after TRRAP overexpression in A2780-AD cells. (C) Luciferase reporter assay of human NANOG, OCT4, and SOX2 promoters after overexpression of control or FLAG-tagged TRRAP in A2780-AD cells (right panel). The expression levels of FLAG-TRRAP and GAPDH were determined through Western blotting analysis with anti-FLAG and anti-GADPH antibodies (left panel). Results are presented as mean ± SD. *P < 0.05 (n = 4).