Fig. 3.

Increase in cordycepin-induced anti-invasiveness by the inhibition of EP4 signaling in HCT-116 cells. (A, B) Cells were pretreated with LPS (2.5 μg/ml) for 1 h prior to treatment with AH23848 or cordycepin (50 μg/ml) for 48 h. The cells were lysed, and equal amounts of protein were subjected to Western blotting. (C, D) Cells were pretreated with LPS (2.5 μg/ml) for 1 h before being challenged with AH23848 or cordycepin (50 μg/ml) for 6 h. The cell migration (C) and Matrigel invasion (D) assays were performed. The experiment was repeated three times, and similar results were obtained.