Table 1.

Primers and probes used.

Reaction	Primers (5’ to 3’)	DNA size (base pairs)
Mouse genotyping		WT	KO
WT α	For: TCCCCTGCGCCACTCTGCACTTGCTT	452	none
KO α	For: TCGATTCCAAACCAGTGTCCTACTATA	(2543)	593
common	Rev: ATTGTTGACGGAAATGTAGAAAGCTTC
common	For: TGTGCTTTGCGGTAGGGCTTCTGTGTA
WT β/γ	Rev: GCTTAGGCGCTCCTTTCTCA	1003	none
KO β/γ	Rev: TTCTGGGCAGCAGATCTTTCCTGCTCT	(3863)	643
WT β	For: TGTGCTTTGCGGTAGGGCTTCTGTGTA	1003	459
and KO β	Rev: GCTTAGGCGCTCCTTTCTCA
WT γ	For: CCTTGCTTCTATACATCATCTCTTAACAGC	153	none
KO γ	For: CCTTGCTTCTATACATCATCTCTTAATAT	none	154
common	Rev: GCTTAGGCGCTCCTTTCTCA
RT-PCR for mouse TFPI isoform transcripts
probe	FAM-CATCGTGGTTCCCCAGTCTC-MGBNFQ
common	For: CTGTGAGAATCCAGTCCACTCC
α primer	Rev: GCCACGATAATCCCGACG
β primer	Rev: TCTTCTTTTGTAACCCGACGC
γ primer	Rev: TACCAAGGCAGCCCGAC
RT-PCR for human γ sequence in transcripts
	For: CAAGAACATTTGTGAAGATGGTCC
	Rev: ATAAAGCTGATGTAGTTCCAAGGC