A. gRNA cloning and sequencing
bnl-lexA gRNA fwd	TATATAGGAAAGATATCCGGGTGAACTTCgTGTATCTGCGAT GCCCCTCAGTTTTAGAGCTAGAAATAGCAAG
bnl-lexA gRNA rev	ATTTTAACTTGCTATTTCTAGCTCTAAAACTCCCGCAATATCTGAAGG ATcGACGTTAAATTGAAAATAGGTC
T3 primer used for sequencing	CAATTA ACCCTCACTAAAGG-3'
Note: nucleotides underlined anneal to U6 promoter or gRNA core on pCFD4 vector, the lowercase g/c was added to aid U6 promoter-dependent transcription
B. HDR donor construction
bnl N-F_pUC19	AATTCGAGCTCGGTACtgtggtctttgaggctggaac
bnl-lexA-N-R	tCCGcaagtCagtAGgctgccgcgtccttcgccggaGCCCGCAGATACAAGGCCC C
lexA-F	CTactGacttgCGGaGAtGTcGAaGAGAACCCtGGCCCtATGCCACCCAA GAAGAAGC
lexA-R	CTAAACGAGTTTTTAAGCAAACTCACTC
bnl lexA-C Fwd	TAAAAACTCGTTTAGACGGGATGGCGTTGTCAAC
bnl C-R_pUC19	GCCAAGCTTGCATGCCtcgcataattgccgcctgg
Note: nucleotides in capital overlap with pUC19 vector for Gibson Assembly, nucleotides underlined were sequence overhang for T2A peptide addition.
C. HDR screening and sequencing
bnl-lexA scr fwd1	GTGGCGCACGCCCAATAAAC
bnl-lexA scr rev1	GATCCCAGCCAATCTCCGTTG
bnl-lexA scr fwd2	CAACGGAGATTGGCTGGGATC
bnl-lexA scr rev2	CTGGCCAACTGTAGGGAAGTC
ends-in check rev3	GCAATGTTATGCAATGCGTTGAC
bnl-lexA seq fwd3	CACTTGTCGCCCATATTGATACAATTG
NOTE: These primers were used for PCR screening and sequencing, the approximate locations of primer binding sites are shown in Figure 5A as fwd1-2 and rev1-3.
D. RT-PCR analysis
RT-f	GATATGGATTTCTCCGCTTTGCTG
RT-r	CCATGCAGAGATACAGGCAAGTG