Table 1. Steep gradient for LC/MS analysis.
| Time (min) | 0.1% formic acid in water | Acetonitrile | Flow rate (μL/min) |
|---|---|---|---|
| 0 | 70 | 30 | 500 |
| 2.0 | 70 | 30 | 500 |
| 5.0 | 5 | 95 | 500 |
| 6.0 | 5 | 95 | 500 |
| 6.5 | 95 | 5 | 500 |
| 8.0 | 95 | 5 | 500 |
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