Table 2. Shallow gradient for LC/MS analysis.
| Time (min) | 0.1% formic acid in water | Acetonitrile | Flow rate (μL/min) |
|---|---|---|---|
| 0 | 95 | 5 | 500 |
| 5 | 95 | 5 | 500 |
| 45 | 5 | 95 | 500 |
| 50 | 5 | 95 | 500 |
| 52 | 95 | 5 | 500 |
| 60 | 95 | 5 | 500 |
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