Table 1. Protein identification by MS.
Spot # | Description | Short name | P | Sc | MW | AC | PEP |
---|---|---|---|---|---|---|---|
1 | Protein disulfide-isomerase | PDI | 1.33E-15 | 200.31 | 56427.9 | 1709617 | 73 |
2 | Protein disulfide-isomerase | PDI | 2.66E-14 | 160.30 | 56427.9 | 1709617 | 27 |
3 | Protein disulfide-isomerase | PDI | 1.00E-30 | 90.29 | 56427.9 | 1709617 | 11 |
3 | Hsp70 | Hsp170 | 8.27E-13 | 20.20 | 66975.0 | 476003 | 2 |
4 | Protein disulfide-isomerase | PDI | 1.38E-07 | 30.16 | 56427.9 | 1709617 | 4 |
4 | Beta-amylase | BAM | 9.90E-05 | 20.14 | 59609.5 | 113786 | 2 |
5 | Protein disulfide-isomerase | PDI | 2.33E-10 | 20.23 | 56427.9 | 1709617 | 2 |
6 | Disease resistance protein homologue | NBS-LRR | 1.20E-02 | 100.10 | 152620.4 | 28555892 | 39 |
7 | Putative transcription repressor HOTR | HOTR1 | 1.00E+00 | 24.12 | 60282.3 | 3550436 | 3 |
7 | Mildew resistance locus a protein 6 | MLA6 | 1.00E+00 | 10.15* | 107751.0 | 12957124 | 9 |
7 | Mildew resistance locus a protein 1 | MLA1 | 1.00E+00 | 10.15* | 108539.5 | 11612210 | 9 |
7 | Mildew resistance locus a protein 6–2 | MLA6-2 | 1.00E+00 | 10.15* | 26749.2 | 33943715 | 9 |
8 | Elongation factor 1A (Wheat) | EF1A | 6.02E-04 | 20.17 | 49137.9 | 399414 | 2 |
8 | RNA polymerase alpha subunit | RPOA | 6.40E-03 | 52.10 | 38880.3 | 118430418 | 24 |
9 | UTP-glucose-1-phosphate uridylyltransferase | UDPGP | 1.00E-30 | 30.28 | 51612.3 | 6136111 | 3 |
9 | Protein disulfide isomerase | PDI | 5.33E-09 | 50.23 | 56427.9 | 1709617 | 5 |
9 | Hypothetical protein | ||||||
9 | ADP-glucose pyrophosphorylase small subunit 1a | AGP-S1a | 1.96E-06 | 20.17 | 51977.6 | 1143500 | 2 |
9 | ADP-glucose pyrophosphorylase small subunit 1b | AGP-S1b | 1.96E-06 | 20.17 | 51962.6 | 51556842 | 2 |
10 | Mildew resistance locus a protein 6 | MLA6 | 1.00 | 10.10* | 107751 | 12957124 | 2 |
10 | Mildew resistance locus a protein 1 | MLA1 | 1.00 | 10.10* | 108540 | 11612210 | 2 |
10 | Mildew resistance locus a protein 6–2 | MLA6-2 | 1.00 | 10.10* | 26749 | 33943715 | 2 |
11 | Mildew resistance locus a protein 6 | MLA6 | 1.00 | 10.20* | 107751 | 12957124 | 7 |
11 | Mildew resistance locus a protein 1 | MLA1 | 1.00 | 10.20* | 108540 | 11612210 | 7 |
11 | Mildew resistance locus a protein 6–2 | MLA6-2 | 1.00 | 10.20* | 26749 | 33943715 | 7 |
12 | UTP-glucose-1-phosphate uridylyltransferase | UDPGP | 1.33E-15 | 120.29 | 51612.3 | 6136111 | 15 |
12 | ADP-glucose pyrophosphorylase small subunit 2 | AGP-S2 | 3.73E-08 | 20.20 | 54745.0 | 27464770 | 2 |
14 | UTP-glucose-1-phosphate uridylyltransferase | UDPGP | 7.29E-09 | 40.17 | 51612.3 | 6136111 | 8 |
15 | Protein disulfide-isomerase | PDI | 2.04E-09 | 120.24 | 56427.9 | 1709617 | 20 |
15 | Beta-amylase | 1.70E-07 | 40.18 | 59609.5 | 113786 | 4 | |
16 | UTP-glucose-1-phosphate uridylyltransferase | UDPGP | 1.01E-08 | 60.19 | 51612.3 | 6136111 | 14 |
16 | Xylose isomerase | XYLA | 2.44E-07 | 10.18 | 53549.6 | 1296807 | 2 |
18 | protein z-type serpin | SrpZ | 1.77E-08 | 40.17 | 43193.4 | 1310677 | 14 |
19 | Glutamate decarboxylase | GAD | 6.55E-07 | 40.17 | 54290.8 | 31296711 | 8 |
19 | Alanine aminotransferase 2; | ALAAT2 | 2.52E-05 | 10.11* | 52844.2 | 1703227 | 2 |
19 | Glucose-1-phosphate adenylyltransferase large subunit 1 | AGP-L1 | 5.01E-05 | 40.16 | 57896.0 | 1707923 | 8 |
19 | ADP-glucose pyrophosphorylase large subunit | AGP-L | 8.96E-04 | 20.12 | 21231.5 | 4467847 | 4 |
20 | Protein disulfide-isomerase | PDI | 1.11E-16 | 180.31 | 56427.9 | 1709617 | 32 |
21 | ADP-glucose pyrophosphorylase small subunit 2 | AGP-S2 | 9.75E-12 | 80.22 | 54745.0 | 27464770 | 8 |
21 | Glucose-1-phosphate adenylyltransferase small subuinit | AGP-S | 9.75E-12 | 40.22 | 56013.8 | 1707940 | 4 |
22 | UTP-glucose-1-phosphate uridylyltransferase | UDPGP | 9.19E-13 | 110.22 | 51612.3 | 6136111 | 26 |
22 | Alpha tubulin 5 | TUB5 | 8.14E-11 | 10.19* | 27953.7 | 146760205 | 2 |
22 | ADP-glucose pyrophosphorylase small subunit 2 | AGP-S2 | 7.81E-10 | 10.20* | 54745.0 | 27464770 | 2 |
22 | Xylose isomerase | XYLA | 7.87E-09 | 50.19 | 53579.7 | 6175480 | 10 |
22 | Glutamate decarboxylase | GAD | 1.27E-05 | 30.19 | 54290.8 | 31296711 | 6 |
23 | Glutamate decarboxylase | GAD | 3.08E-07 | 20.13 | 54290.8 | 31296711 | 4 |
23 | AF100770_1 receptor-like kinase | 2.22E-05 | 10.14* | 45074.1 | 5669672 | 2 | |
23 | Beta-amylase | BAM | 4.27E-05 | 10.12* | 59609.5 | 113786 | 2 |
24 | Glucose-1-phosphate adenylyltransferase large subunit 1 | AGP-L1 | 2.65E-09 | 30.25 | 57896.0 | 1707923 | 3 |
24 | Beta-amylase | 3.43E-08 | 50.19 | 59609.5 | 113786 | 7 | |
24 | AF414081_1 endosperm-specific beta-amylase 1 | BAM-en | 3.43E-08 | 50.19 | 59534.3 | 29134855 | 7 |
P: probability that the protein identification was random (lower number = better identification); Score: scoring used in identification algorithm; MW: molecular weight reported in accession number; AC: NCBI accession number; PEP: number of peptides identified for the protein