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. 2018 Nov 2;16(11):e3000047. doi: 10.1371/journal.pbio.3000047

Fig 1. GSDMD cleavage correlates with IL-1β secretion and pyroptosis.

Fig 1

BMMs were isolated from WT, Gsdmd−/−, Nlrp3−/−, Casp1−/−, Casp11−/−, NOMID, or NOMID;Gsdmd−/− mice and were expanded in vitro in the presence of M-CSF-containing media. BMMs were primed with LPS for 3 hours and treated with nigericin for 30 minutes. (A, D) Western blot analysis of cell lysates. (B, E) IL-1β levels in conditioned media. (C, F) Percent of LDH release in conditioned media. Data are mean ± SEM from experimental triplicates and are representative of at least three independent experiments. The numerical values underlying Fig 1B, 1C, 1E and 1F can be found in S1 Data. ****P < 0.0001. BMM, bone marrow–derived macrophage; Casp, caspase; GSDMD, gasdermin D; IL-1β, interleukin-1β; LDH, lactate dehydrogenase; LPS, lipopolysaccharide; M-CSF, macrophage colony-stimulating factor; NOMID, neonatal-onset multisystem inflammatory disease; WT, wild-type.