Fig 4. GSDMD deficiency prevents damage to multiple tissues in NOMID mice.

Representative images of liver, skin, and spleen from 3-week-old WT mice (3 males and 1 female), NOMID mice (3 males and 1 female), NOMID;Gsdmd^−/− mice (2 males) and Gsdmd^−/− mice (3 males and 1 female) stained with HE. Femurs were stained for TRAP activity. Red circle indicates area of hypocellularity in the epiphysis. Osteoclasts are stained in red. Scale bar: 200 μm. GSDMD, gasdermin D; HE, hematoxylin–eosin; NOMID, neonatal-onset multisystem inflammatory disease; TRAP, tartrate-resistant acid phosphatase; WT, wild-type.