| Serial dilute as follows (starting with the stock solution) | ||||||
| Barcoding reagent | Stock concentration | #1 | #2 | #3 | #4 | unstained |
| Alexa Fluor 488 | 10 mg/mL | 1:500 | 1:5 | x | ||
| Pacific Blue | 10 mg/mL | 1:2500 | 1:4 | 1:4 | 1:10 | |
| Pacific Orange | 2 mg/mL | 1:50 | 1:12 | 1:24 |
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