Figure 1: HUMAnN2 functionally profiles microbial communities with high accuracy using tiered search.
(a) Overview of HUMAnN2’s tiered search algorithm for meta’omic functional profiling (expanded in Supplementary Fig. 1). (b) HUMAnN2’s tiered search vs. pure translated search evaluated on a synthetic gut metagenome. Sensitivity, precision, and overall accuracy (1 - Bray-Curtis dissimilarity) were computed for (c) gene family and (d) pathway abundance profiles relative to gold standards at the whole-community level (“overall”) and for each stratification. (e) HUMAnN2 compared with other methods in the task of quantifying community-total COG abundances. Runtimes reflect multi-threaded execution on 8 CPU cores.