Table 1.
The primary and secondary antibodies used in this study
| Antibody | Immunogen | Source, host and clonality, cat #, RRID | Dilution |
|---|---|---|---|
| Primary antibody | Primary antibody dilution | ||
| Rho4D2 | Bovine (Bos taurus) rhodopsin: NGTEGPNFYVPFS NKTGVVRSPFEAP QYYLAEPWQFSM |
Abcam, mouse monoclonal, cat # ab98887, RRID:AB_2315274 | 1:500 |
| Ret-P1 | Rat (Rattus norvegicus) retina membrane fraction, epitope - rhodopsin: TEGPNFY |
Millipore, mouse monoclonal, cat # MAB5316, RRID:AB_2156055 |
1:300 |
| OPN1SW | Human (Homo sapiens) blue-sensitive opsin: EFYLFKNISSVGP WDGPQYH |
Santa Cruz, goat polyclonal, cat # SC14363, RRID:AB_2158332 | 1:300 |
| Secondary antibody | Secondary antibody dilution | ||
| Goat-antimouse IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugated | Gamma Immunoglobins Heavy and Light chains | Life Technologies, goat polyclonal, cat # A-11001, RRID:AB_2534069 | 1:1000 |
| Donkey-anti-goat IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugated | Gamma Immunoglobins Heavy and Light chains | Life Technologies, donkey polyclonal, cat # A-11055, RRID:AB_2534102 | 1:1000 |