| Gene | Primer sequence | Annealing temperature |
| MMITS1 | F: CACCACGAGAAACACTCCAA | 65 |
| 16S–23SITS Locus AB548718 for M. marinum quantification | R: ACATCCCGAAACCAACAGAG | |
| loopern4 | F: TGAGCTGAAACTTTACAGACACAT | 61 |
| Expressed repetitive elements | R: AGACTTTGGTGTCTCCAGAATG | |
| il4 | GCAGGAATGGCTTTGAAGGG | 59.5 |
| ZDB-GENE-100204-1 | GCAGTTTCCAGTCCCGGTAT | |
| ifnγ1-2 | F: GGGCGATCAAGGAAAACGACCC, | 61 |
| ZDB-GENE-040629-1 | R: TAGCCTGCCGTCTCTTGCGT |
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