Name of Material/ Equipment	Stock Concentration	Final Concentration	Volume / Amount
Detergent Lysis Buffer
Low sucrose buffer	-	-	600 μL
Triton-X	20%	0.10%	3 μL
Hypotonic Lysis buffer
Tris-HCl (pH = 7.4)	1 M	10 mM	100 μL
NaCl	5 M	10 mM	20 μL
MgCl2	1 M	3 mM	30 μL
Nonidet P40	-	0.01%	1 μL
Nuclease-free water			up to 10 mL
HEB Medium
Hibernate-A	-	-	10 mL
Glutamax	-	-	100 μL
B27	-	-	200 μL
Low sucrose buffer
Sucrose	-	0.32 M	2.75 g
HEPES (pH = 8.0)	1 M	10 mM	250 μL
CaCl2	1 M	5 mM	125 μL
MgAc	1 M	3 mM	75 μL
EDTA	0.5 M	0.1 mM	5 μL
DTT	1 M	1 mM	25 μL
Nuclease-free water			up to 25 mL
Sucrose density buffer
Sucrose	-	1 M	8.6 g
HEPES (pH = 8.0)	1 M	10 mM	250 μL
MgAc	1 M	3 mM	75 μL
DTT	1 M	1 mM	25 μL
Nuclease-free water			up to 25 mL
Resuspension Solution
1X PBS	-	-	1 mL
BSA	20 mg/mL	0.4 mg/mL	20 μL
RNAse Inhibitor	40 U/μL	0.2 U/μL	5 μL