Reagent	Final Concentration	Volume for PCR1 plate (µL)	Volume for PCR2 plate (µL)
Forward Primer	1 µM	32.3	23.8
Reverse Primer	1 µM	32.3	23.8
Buffer (10x)	1x	323	238
MgSO4 (50 mM)	2 mM	129.2	95.2
dNTP (10 mM)	0.2 mM	64.6	47.6
DNA polymerase (5 U/µL)	0.025 U/µL	16.2	11.9
Ultrapure H2O		2632.5	1939.7