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. 2018 Nov 14;4(11):eaau9859. doi: 10.1126/sciadv.aau9859

Fig. 3. AAV9-mediated gene transfer in the brain is predominately targeted to neurons.

Fig. 3

(A to D) Intrathecal AAV9 infusion and inversion for 2 hours: (A) 95.5% of GFP-labeled cells are neurons, by colocalization with neuronal nuclei (NeuN); (B) 0.779% of GFP-labeled cells are astrocytes, by colocalization with glial fibrillary acidic protein (GFAP) and sex-determining region Y-box9 (Sox9); and (C) 4.39% of GFP-labeled cells are oligodendrocytes, by colocalization with adenomatous polyposis coli (APC). (D) No microglia were transduced by colocalization with ionized calcium-binding adapter molecule 1 (Iba1). Scale bars, 50 μm.