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. 2018 Oct 11;7:e37960. doi: 10.7554/eLife.37960

Figure 4. Graphical representation of the model for carbon fixation in Chlamydomonas reinhardtii.

The model includes a copy of the Calvin-Benson cycle (CBC) in the chloroplast stroma and in the pyrenoid. In addition, the model considers reversible transport between the stroma and the pyrenoid for all CBC intermediates. A complete list of enzyme and metabolite names corresponding to the given abbreviations is presented in Supplementary file 3.

Figure 4.

Figure 4—figure supplement 1. Distribution of Chi-square statistic for 5000 sampled steady-state flux distributions under three different assumptions.

Figure 4—figure supplement 1.

Model predictions under non-CCM (HC, (A) and CCM-induced (LC*; B) conditions were validated using the Chi-square statistic between predicted and measured total concentration for 11 metabolites over 5000 sampled steady-state flux distributions. The validation was done under three different assumptions: (i) activity of PRK, Rubisco and GAPDH, (ii) activity of PRK and Rubisco, and (iii) only activity of Rubisco in the pyrenoid. The red line shows the Chi-square statistic corresponding to a significance level of 0.05.