Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (C. neoformans species complex) |
XL280, MATα, wild-type | PMID: 17112316 |
||
| Genetic reagent (C. neoformans species complex) |
XL280, MATa, wild-type | PMID: 23670559 | ||
| Genetic reagent (C. neoformans
species complex) |
JEC21, MATα, wild-type | PMID: 10512666 | ||
| Genetic reagent (C. neoformans species complex) | JEC20, MATa, wild-type | PMID: 10512666 | ||
| Genetic reagent (C. neoformans
species complex) |
KN99, MATα, wild-type | PMID: 12933823 | ||
| Genetic reagent (C. neoformans species complex) | KN99, MATa, wild-type | PMID: 12933823 | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα, CSA2::NEOr | This study | See Materials and methods, ‘Gene disruption and overexpression’ |
|
| Genetic reagent (C. neoformans species complex) |
XL280, MATa, CSA2::NEOr | This study | ||
| Genetic reagent(C. neoformans species complex) | H99, MATα, CSA2::NEOr | This study | ||
| Genetic reagent (C. neoformans species complex) |
H99, MATa, CSA2::NEOr | This study | ||
| Genetic reagent (C. neoformans species complex) |
JEC21, MATα, CSA2::NEOr | This study | ||
| Genetic reagent (C. neoformans species complex) |
JEC20, MATa, CSA2::NEOr | This study | ||
| Genetic reagentC. neoformans
species complex) |
XL280, MATα, FAD1::NEOr | PMID: 24901238 | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATa, FAD1::NEOr | This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα, PUM1::NEOr | PMID: 24901238 | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATa, PUM1::NEOr | PMID: 24901238 | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα, CSA1::NATr | This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATa, CSA1::NATr | This study | ||
| Genetic reagent (C. neoformans species complex) |
H99, MATα, CSA1::NEOr | This study | ||
| Genetic reagent (C. neoformans species complex) |
H99, MATa, CSA1::NEOr | This study | ||
| Genetic reagent (C. neoformans species complex) |
JEC21, MATα, CSA1::NEOr | This study | ||
| gGenetic reagent (C. neoformans species complex) |
JEC20, MATa, CSA1::NEOr | This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα, DMC1::NEOr | This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα, SPO11::NEOr | PMID: 23966871 | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα, CSA2::NEOr,
CSA1::NATr |
This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATa, CSA2::NEOr,
CSA1::NATr |
This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα,
PRPBL2B-PUM1-HYG |
This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα,
PDMC1-DMC1-mCherry-3'UTR-HYG |
PMID: 24901238 | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATa, PDMC1-DMC1-
mCherry-3'UTR-HYG |
This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα, CSA2::NEOr, PDMC1-
DMC1-mCherry-3'UTR-HYG |
This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα,
CSA1::NATr, PDMC1- DMC1-mCherry-3'UTR-HYG |
This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα,
PFAD1-FAD1-mCherry- HYG |
This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα,
CSA2::NEOr, PFAD1-FAD1-mCherry-HYG |
This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα,
PFAS1-FAS1-mCherry-HYG |
This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα,
PDHA1-DHA1- mCherry-HYG |
This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα,
CSA1::NATr, PFAD1- FAD1-mCherry-HYG |
This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα, CSA1::NATr, PFAD1-
FAD1-mCherry-HYG |
This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα, CSA1::NATr,
CSA2::NEOr, PDMC1-DMC1-mCherry-3'UTR-HYG |
This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα, CSA1::NATr,
CSA2::NEOr, PFAD1-FAD1-mCherry-3'UTR-HYG |
This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα, CNB02310::NEOr | This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα, CNF03810::NEOr | This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα,
CNF01980::NEOr |
This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα, CNB05180::NEOr | This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα, CNF00260::NEOr | This study | ||
| Genetic reagent (C. neoformans species complex) |
XL280, MATα, CNG01790::NEOr | This study | ||
| Software, algorithm |
RStudio Version 1.1.456 | RStudio | RRID:SCR_000432 | |
| Software, algorithm |
FastQC v0.11.5 | RRID:SCR_014583 | ||
| Software, algorithm |
DEseq2 v1.16.1 | RRID:SCR_016533 | ||
| Software, algorithm |
Hisat2 v2.1.0 | RRID:SCR_015530 | ||
| Software, algorithm |
BiNGO v3.0.3 | RRID:SCR_005736 | ||
| Software, algorithm |
Graphpad Prism 6 | Graphpad | RRID:SCR_002798 | |
| Sequence- based reagent |
Wanglab959 (knockout primer pairs) |
This study | TTGTCACCAACCTATCCGCTAC | |
| Sequence- based reagent |
Wanglab960 (knockout primer pairs) |
This study | CAGTTTGCTCTTATTCCCACTCC | |
| Sequence- based reagent |
Wanglab961 (knockout primer pairs) |
This study | CTGGCCGTCGTTTTACGAAGCACTTGGTGAATGAGACATT | |
| Sequence- based reagent |
Wanglab962 (knockout primer pairs) |
This study | GTCATAGCTGTTTCCTGCACCGCCCTTACGATTATACATCT | |
| Sequence- based reagent |
Wanglab963 (knockout primer pairs) |
This study | GTTGTGAGTGTCATGAGTGTCATTG | |
| Sequence- based reagent |
Wanglab964 (knockout primer pairs) |
This study | CCTCTTCTGCCAATAACCCTTTT | |
| Sequence- based reagent |
Wanglab2195 (knockout primer pairs) |
This study | CATCCCCAGAACACGCTGAT | |
| Sequence- based reagent |
Wanglab2196 (knockout primer pairs) |
This study | TCCGGCCATTAAGATCCGTG | |
| Sequence- based reagent |
Wanglab2197 (knockout primer pairs) |
This study | AAAACGGCAACAGTCAAGGC | |
| Sequence- based reagent |
Wanglab2198 (knockout primer pairs) |
This study | CTGGCCGTCGTTTTACGTTGTTAAAGGCAGTTGAGCGA | |
| Sequence- based reagent |
Wanglab2199 (knockout primer pairs) |
This study | GTCATAGCTGTTTCCTGAAGGCATCACTTCGTTTGGC | |
| Sequence- based reagent |
Wanglab2200 (knockout primer pairs) |
This study | AACCATAGGATGTGCCACGC | |
| Sequence- based reagent |
Wanglab953 (knockout primer pairs) |
This study | CCGTAGGCTTATCCCAGTCAGA | |
| Sequence- based reagent |
Wanglab954 (knockout primer pairs) |
This study | GTGGAAGGCAAGAGTTGGTGTT | |
| Sequence- based reagent |
Wanglab955 (knockout primer pairs) |
This study | CTGGCCGTCGTTTTACACATTTCCAGAAGAGGCAAGAAGA | |
| Sequence- based reagent |
Wanglab956 (knockout primer pairs) |
This study | GTCATAGCTGTTTCCTGGGGTAGAAGAACGTCAAACAACTAA | |
| Sequence- based reagent |
Wanglab957 (knockout primer pairs) |
This study | CCTTGGCAACAGTAGGCTTCTG | |
| Sequence- based reagent |
Wanglab958 (knockout primer pairs) |
This study | GGAAGGGAGTGGTGAGGTTGAA | |
| Sequence- based reagent |
Wanglab2461 (knockout primer pairs) |
This study | GGGCCTGAAAAGTATGAAGTCC | |
| Sequence- based reagent |
Wanglab2462 (knockout primer pairs) |
This study | TAGCCTTTCCACCACAGCAGC | |
| Sequence- based reagent |
Wanglab2463 (knockout primer pairs) |
This study | CTGGCCGTCGTTTTACGTAGCGGTTTCGACGGACATAT | |
| Sequence- based reagent |
Wanglab2464 (knockout primer pairs) |
This study | GTCATAGCTGTTTCCTGGGAAGAGGAGGAGACCAAGGAG | |
| Sequence- based reagent |
Wanglab2465 (knockout primer pairs) |
This study | ATCCTTTGTCCAACCCGTGAG | |
| Sequence- based reagent |
Wanglab2466 (knockout primer pairs) |
This study | GCCCATGTCGCATTACGTAAAG | |
| Sequence- based reagent |
Wanglab2423 (knockout primer pairs) |
This study | AGCCATTCGGCTCTTATCGC | |
| Sequence- based reagent |
Wanglab2424 (knockout primer pairs) |
This study | AGCGACTGCGACCATTATGT | |
| Sequence- based reagent |
Wanglab2425 (knockout primer pairs) |
This study | CTGGCCGTCGTTTTACATGGAGGCGTTGGAGAATCC | |
| Sequence- based reagent |
Wanglab2426 (knockout primer pairs) |
This study | GTCATAGCTGTTTCCTGGCAAGACGTGCATACCCTCTA | |
| Sequence- based reagent |
Wanglab2427 (knockout primer pairs) |
This study | GCTTCAGTATGCCAACCCCT | |
| Sequence- based reagent |
Wanglab2428 (knockout primer pairs) |
This study | CGAGAGAAGGGAAAGCGAGG | |
| Sequence- based reagent |
Wanglab2201 (knockout primer pairs) |
This study | GGAGAGATCAGAGGCAGCAC | |
| Sequence- based reagent |
Wanglab2202 (knockout primer pairs) |
This study | CGTCGTGGAAAAGGTGAGGA | |
| Sequence- based reagent |
Wanglab2203 (knockout primer pairs) |
This study | TCCGGATTTCTCAAGTGGGC | |
| Sequence- based reagent |
Wanglab2204 (knockout primer pairs) |
This study | CTGGCCGTCGTTTTACGCT CTAGCATTTGCGGGGAT |
|
| Sequence- based reagent |
Wanglab2205 (knockout primer pairs) |
This study | GTCATAGCTGTTTCCTGTGACTCCCCCTCCAGAAAGC | |
| Sequence- based reagent |
Wanglab2206 (knockout primer pairs) |
This study | AACCAAAATGGCTCCGGACA | |
| Sequence- based reagent |
Wanglab2682 (knockout primer pairs) |
This study | TTGCAACCATCCGAGGTCAA | |
| Sequence- based reagent |
Wanglab2683 (knockout primer pairs) |
This study | GAAATCCGACACCTCCCTGG | |
| Sequence- based reagent |
Wanglab2684 (knockout primer pairs) |
This study | CTGGCCGTCGTTTTACGG GATGTTTGTCCCTTTCGC |
|
| Sequence- based reagent |
Wanglab2685 (knockout primer pairs) |
This study | GTCATAGCTGTTTCCTGACCAGTAAGAAGCGGTGACA | |
| Sequence- based reagent |
Wanglab2686 (knockout primer pairs) |
This study | AGCGCTCGACTAGCTTTCTC | |
| Sequence- based reagent |
Wanglab2687 (knockout primer pairs) |
This study | GGATCCAAGACCTCCGATGG | |
| Sequence- based reagent |
Wanglab3060 (knockout primer pairs) |
This study | AGCGATAAGCCAGCAAGAGTT | |
| Sequence- based reagent |
Wanglab3061 (knockout primer pairs) |
This study | CCTCGAACCCGATACTGACG | |
| Sequence- based reagent |
Wanglab3062 (knockout primer pairs) |
This study | AGCTTAGAATAGCGACCGCC | |
| Sequence- based reagent |
Wanglab3063 (knockout primer pairs) |
This study | CTGGCCGTCGTTTTACTGTGA GAGTCGGCTGATAGGA |
|
| Sequence- based reagent |
Wanglab3064 (knockout primer pairs) |
This study | GTCATAGCTGTTTCCTGGTGGAACCTAATTGCACCGC | |
| Sequence- based reagent |
Wanglab3065 (knockout primer pairs) |
This study | ATGGCGAGTTGCTTTCATGC | |
| Sequence- based reagent |
Wanglab3066 (knockout primer pairs) |
This study | TAATGTCGCTGAAGGGCCTG | |
| Sequence- based reagent |
Wanglab3067 (knockout primer pairs) |
This study | CCAAGGGTCAGCTATCCAGC | |
| Sequence- based reagent |
Wanglab3068 (knockout primer pairs) |
This study | CCGTAACCGGTGAGACATCA | |
| Sequence- based reagent |
Wanglab3069 (knockout primer pairs) |
This study | CTGGCCGTCGTTTTACGAGACGAATGAGCTGTGGCA | |
| Sequence- based reagent |
Wanglab3070 (knockout primer pairs) |
This study | GTCATAGCTGTTTCCTGTCAA GTCATGCCTGTGATCCT |
|
| Sequence- based reagent |
Wanglab3071 (knockout primer pairs) |
This study | AGATCCTGGAGGGAACGGAT | |
| Sequence- based reagent |
Wanglab3072 (knockout primer pairs) |
This study | TTAGCTCGCCCTCGCTTATT | |
| Sequence- based reagent |
Wanglab3073 (knockout primer pairs) |
This study | AGCCAACCCATTTACCGACT | |
| Sequence- based reagent |
Wanglab3074 (knockout primer pairs) |
This study | CGTTGGACAATGGAGTGAGGA | |
| Sequence- based reagent |
Wanglab3075 (knockout primer pairs) |
This study | CTGGCCGTCGTTTTACGGGGA TGAAGGGAGCTAAAGG |
|
| Sequence- based reagent |
Wanglab3076 (knockout primer pairs) |
This study | GTCATAGCTGTTTCCTGGAAG CCTTTGCATTTGACCCT |
|
| Sequence- based reagent |
Wanglab3077 (knockout primer pairs) |
This study | GGACAGAGGCCGTCAACATA | |
| Sequence- based reagent |
Wanglab3646 (knockout primer pairs) |
This study | CTAACGACAACAAGAAACCACGAC | |
| Sequence- based reagent |
Wanglab3647 (knockout primer pairs) |
This study | CTGGCCGTCGTTTTACAGGCGGA GGAAGGTAGGAGAA |
|
| Sequence- based reagent |
Wanglab3648 (knockout primer pairs) |
This study | GTCATAGCTGTTTCCTGGTAGGTAA TGTTGACGGTGGTGA |
|
| Sequence- based reagent |
Wanglab3649 (knockout primer pairs) |
This study | GTCTTAGTGGTCTGAGCCGAATAC | |
| Sequence- based reagent |
Wanglab3650 (knockout primer pairs) |
This study | AGGACGCTATTCGCTCTATCGG | |
| Sequence- based reagent |
Wanglab3651 (knockout primer pairs) |
This study | GATCCTTCACCCTGACTCTGTTCA | |
| Sequence- based reagent |
Wanglab3261 (knockout primer pairs) |
This study | ACTCATGCCTACCCATTGCC | |
| Sequence- based reagent |
Wanglab3262 (knockout primer pairs) |
This study | GCGACTCACTGAGCTTGACA | |
| Sequence- based reagent |
Wanglab3263 (knockout primer pairs) |
This study | CGGGCTTTACACCTACTCGG | |
| Sequence- based reagent |
Wanglab3264 (knockout primer pairs) |
This study | CTGGCCGTCGTTTTACTCTGC TTGTACGTCAGCGAT |
|
| Sequence- based reagent |
Wanglab3265 (knockout primer pairs) |
This study | GTCATAGCTGTTTCCTGAGTGA AGAGACTTGACGCTCG |
|
| Sequence- based reagent |
Wanglab3266 (knockout primer pairs) |
This study | ACTAGCCCGAAGTGATGGGA | |
| Sequence- based reagent |
Wanglab3267 (knockout primer pairs) |
This study | GGCGCGTTGTAAAGCAGTAG | |
| Sequence- based reagent |
Wanglab3268 (knockout primer pairs) |
This study | TCTCCCCTCGGAAACAGCTA | |
| Sequence- based reagent |
Wanglab3269 (knockout primer pairs) |
This study | AGCACCTTTGCGATGTCTGA | |
| Sequence- based reagent |
Wanglab3270 (knockout primer pairs) |
This study | CTGGCCGTCGTTTTACGTTC CTGGACCCTTGATCCC |
|
| Sequence- based reagent |
Wanglab3271 (knockout primer pairs) |
This study | GTCATAGCTGTTTCCTGGC AGTAACGGTCCTGTTCCA |
|
| Sequence- based reagent |
Wanglab3272 (knockout primer pairs) |
This study | GTTCGATCAGAAACACGGCG | |
| Sequence- based reagent |
Wanglab857 (qRT-PCR primer) |
This study | CGTCACCACTGAAGTCAAGT | |
| Sequence- based reagent |
Wanglab858 (qRT-PCR primer) |
This study | AGAAGCAGCCTCCATAGG | |
| Sequence- based reagent |
Wanglab3401 (qRT-PCR primer) |
This study | AGACTCGACCACAGGCAG | |
| Sequence- based reagent |
Wanglab3402 (qRT-PCR primer) |
This study | AAAGGACAGGGTCAGGGTT | |
| Sequence- based reagent |
Wanglab2583 (qRT-PCR primer) |
This study | TTCTGCCGTAATGGGTGTCA | |
| Sequence- based reagent |
Wanglab2584 (qRT-PCR primer) |
This study | TCGTAAGGGCGGTGTTGTG | |
| Sequence- based reagent |
Wanglab2585 (qRT-PCR primer) |
This study | GTGAGATTATTGCCCGTGATGA | |
| Sequence- based reagent |
Wanglab2586 (qRT-PCR primer) |
This study | TTGGAGACGCCAGGGATGT | |
| Sequence- based reagent |
Wanglab855 |
This study | CTCTGGTTGGCACGGTG | |
| Sequence- based reagent |
Wanglab856 | This study | CGTCGGTCAATCTTCTCG | |
| Sequence- based reagent |
Wanglab2689 (overexpression primer) |
This study | TTTGCATTGCGGCCGCAGGG GTGAATCGATATTCGACGC |
|
| Sequence- based reagent |
Wanglab2690 (overexpression primer) |
This study | GGATAATTGCGATCGCCAGCTG GAGAGTGACAGACTTGG |