Cell cycle part	Operational Definition	Calculation*	Value**
Progenitor Zone nuclei	all WAPL-1 (or REC-8) positive, HIM-3 negative nuclei	A+B+C+D	231 ± 23 nuclei
S-phase nuclei	nuclei EdU positive after 30 min EdU label and WAPL-1 positive	A+C	133 ± 20 nuclei
M-phase nuclei	pH3 and WAPL-1 co-positive nuclei	A+B	5.2 ± 2.3 nuclei
S-phase index	S-phase nuclei / Progenitor Zone nuclei	A+C/ A+B+C+D	57% of cell cycle
M-phase index	M-phase nuclei / Progenitor Zone nuclei	A+B/ A+B+C+D	2% of cell cycle
Meiotic Entry cells	EdU labeled nuclei in meiosis	E	varies by duration of EdU label
Meiotic Entry rate	Meiotic entry nuclei per h of EdU label	Slope from Figure 4C***	20.3 nuclei per h
G2 duration (median)	50% intercept from Figure4A	2.5 h
G2 duration (maximum)	99% intercept from Figure 4A	3.5 h
G2+M+G1 duration (maximum)	99% intercept from Figure 4B	3.5 h
Cell cycle duration (median)	median G2 duration / G2-index****	6.5 h
Cell cycle duration (maximum)	maximum G2 duration / G2-index****	8.1 h