Sequencing Metrics	Stress1	Control1
(n = 4)	(n = 4)
Paired End Reads (PER)	89,290,397	80,165,674
Uniquely Mapped Paired End Reads (UMPER)	39,200,255	35,013,406
Alignment Rate/Mapping Efficiency (UMPER/PER)	44%	44%
Duplicate Reads (% of UMPER)	73%	65%
Deduplicated UMPER	10,481,031	12,306,018
Average Read Depth Coverage (x) (ARDC)	6x	6x
CpGs (N)	12,056,878	12,056,878
ARDC (x) of CpGs	2x	2x
CpGs with at least 10 reads (N)	481,383	595,850
ARDC (X) of CpGs with at least 10 reads	19	19
On Target CpGs (complete overlap with Probe Target Regions)	1,923,872	2,007,638
On Target ARDC (x) of CpGs	7x	8x
On Target CpGs with at least 10 reads (N)	428,249	531,419
On Target ARDC (x) of CpGs with at least 10 reads	18x	18x
On Target (PER with 1 or more Base Pair overlap with Probe Target Regions) (UMPER)	8,277,715	9,369,523
% On Target (of Deduplicated UMPER)	78%	77%
On Target (Total Bases Mapped) Mb	125 Mb	128 Mb
On Target Average Read Depth Coverage (x) (ARDC)	9x	10x
1Sequencing metrics based on averages across subjects in each group