| Component | Stock conc. | Final reaction conc. | Volume per reaction |
| Taq Polymerase Master Mix | 2x | 1x | 10 µL |
| Primer C1-J-2195 | 20 µM | 0.4 µM | 0.4 µL |
| Primer TL2-N-3014 | 20 µM | 0.4 µM | 0.4 µL |
| Molecular Grade Water | - | - | 8.2 µL |
| DNA Template | - | - | 1 µL |
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