Figure 7.

Developmental Properties of the Reprogrammed Clones

The 1D reprogrammed clone was GFP labeled and injected into stage X-XII recipient embryos as described previously (Aubel and Pain, 2013) to evaluate its developmental potential.

(A) Three injected embryos showing a contribution of the injected cells to various parts of the embryos, including the vitelline membrane, but mass-like structures were almost always observed in the injected embryos, reflecting a lack of in vivo differentiation of the reprogrammed cells. Bright-light embryo observations (upper panel) and corresponding GFP detection (lower panel). Scale bars, 1 mm.

(B) GFP and Hoechst detection by confocal microscopy on embryo cryosections. Scale bars, 50 μm.