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Increased endothelial nitric oxide synthase (eNOS) expression with Rho-associated kinase (ROCK)2 deficiency. Expression of eNOS, p-eENOS, p-MBS, t-MBS, and GAPDH in (A) brain tissues and (B) mouse brain endothelial cells (MBECs) of wild-type (WT), hemizygous ROCK1+/−, hemizygous ROCK2+/− and (C) endothelial cell-specific ROCK2-KO (EC-ROCK2−/−) mice, as assessed by Western blotting (n=3). **P<0.01, ****P<0.0001. All data are expressed as mean±SEM.
