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. Author manuscript; available in PMC: 2018 Nov 15.
Published in final edited form as: Nat Chem. 2018 May 14;10(7):787–794. doi: 10.1038/s41557-018-0055-2

Figure 3. Fluorescent imaging studies of the click reaction between 21 and 22 in Raw264.7 cells.

Figure 3

The click reaction between 21b and 22b resulted in dose-dependent increase in fluorescent intensity, which tracked that of a fluorescent mitochondrion-tracker, while the control click reaction between 21a and 22a only showed negligible fluorescence. Images a, b, c and d show the results from the click reaction between compound 21 and 22. a) RAW 264.7 cells treated with 22a (1 μM) and 21a (1 μM); b) RAW 264.7 cells treated with 22a (5 μM) and 21a (5 μM); c) RAW 264.7 cells treated with 22b (1 μM) and 21b (1 μM); d) RAW 264.7 cells treated with 22b (5 μM) and 21b (5 μM). Images e, f, g and h are confocal images of RAW 264.7 cells treated with compound 21b (5 μM), 22b (2.5 μM) and MT-deep red (50 nM). e) Bright field; f) Red channel; g) DAPI channel; h) Merged images of e), f) and g). Images represent 3 fields of view from 3 independent experiments in triplicate.